Effective stimulation of donors for granulocyte transfusions with recombinant methionyl granulocyte colony-stimulating factor [see comments]

RAPID COMMUNICATION Effective Stimulation of Donors for Granulocyte Transfusions With Recombinant Methionyl Granulocyte Colony-Stimulating Factor

Effective granulocyte transfusion (GT) therapy has been hampered by the low yield of neutrophil granulocytes (PMN) obtainable from normal donors even by use of corticosteroid prestimulation, hydroxyethyl starch (HES), and modern leukapheresis (LA) techniques. To increase the PMN yield we performed LA in 22 healthy volunteer donors after a single subcutaneous administration of 300 fig of granulo...

Effective stimulation of donors for granulocyte transfusions with recombinant methionyl granulocyte colony-stimulating factor.

Effective granulocyte transfusion (GT) therapy has been hampered by the low yield of neutrophil granulocytes (PMN) obtainable from normal donors even by use of corticosteroid prestimulation, hydroxyethyl starch (HES), and modern leukapheresis (LA) techniques. To increase the PMN yield we performed LA in 22 healthy volunteer donors after a single subcutaneous administration of 300 micrograms of ...

Potentiation of neutrophil functions with administration of granulocyte colony-stimulating factor to donors for granulocyte transfusions.

Granulocyte transfusions from granulocyte colony-stimulating factor-treated donors: also a question of cell survival?

Recent articles published in this Journal'.* and one Editorial' focused on granulocyte (PMN) transfusions from donors pretreated with granulocyte colony-stimulating factor (G-CSF) to increase cell numbers. Although the therapeutical value of granulocyte transfusion in infected neutropenic patients is not definitively established, as discussed in the Editorial by Strauss,' the results reported s...

Production of Recombinant Human Granulocyte-Colony Stimulating Factor by Pichia pastoris

Human granulocyte-colony stimulating factor (hG-CSF) cDNA was expressed in the methylotrophic yeast Pichia pastoris under the control of the alcohol oxidase (AOX1) promoter. An expression vector for hG-CSF secretion was constructed using vector pPIC9. Higher levels of hG-CSF was obtained using a P. pastoris Mut+ (methanol utilization fast) phenotype. The effects of environmental factors such as...